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柳树叶浸提液对蛋白核小球藻的化感作用

李庆华;郭沛涌;田美燕;江中央   

  1. 华侨大学环境科学与工程系, 福建厦门 361021
  • 收稿日期:2008-09-03 修回日期:1900-01-01 出版日期:2009-05-10 发布日期:2009-05-10

Allelopathy of Salix babylonica leaf extracts on Chlorella pyrenoidosa.

LI Qing-hua;GUO Pei-yong;TIAN Mei-yan;JIANG Zhong-yang   

  1. Department of Environmental Science and Engineering, Huaqiao University, Xiamen 361021, Fujian, China
  • Received:2008-09-03 Revised:1900-01-01 Online:2009-05-10 Published:2009-05-10

摘要: 通过对蛋白核小球藻进行细胞密度的测定,分析了不同浓度的柳树(Salix babylonica)叶浸提液对蛋白核小球藻(Chlorella pyrenoidosa)生长和细胞形态的影响。结果表明:柳树叶浸提液对蛋白核小球藻具有化感作用,不同浓度的浸提液化感作用强度不同;作用96 h的半效应浓度(EC50,96 h值)为11.82 g·L-1,较低浓度(10和20 g·L-1)浸提液对蛋白核小球藻表现出先抑制后促进的作用特性,而较高浓度(30 g·L-1)浸提液则始终表现出抑制效果;10、20和30 g·L-1浸提液对蛋白核小球藻的最大抑制率分别为37.5%、39.3%和56.5%;柳树叶浸提液作用下,部分蛋白核小球藻细胞平均颗粒体积增大,颜色变透明,膨胀后溶解;低浓度浸提液处理组少数细胞聚集在一起,高浓度浸提液处理组细胞大量聚集,形成多细胞群体,多数沉于瓶底,沉降性能增强。

关键词: 乙草胺, 甲胺磷, 真菌种群, PCR-DGGE, 黑土

Abstract: The allelopathic effects of Salix babylonica leaf extracts on the growth and morphology of Chlorella pyrenoidosa were investigated by measuring the cell density of treated algae. It was shown that the leaf extracts of S. babylonica had definite allelopathic effects on C. pyrenoidosa, depending on their concentration. The EC50, 96 h value of the extracts was 1182 g·L-1. Lower concentrations (10 and 20 g·L-1) of the extracts inhibited the algal growth first and promoted it then, while higher concentration (30 g·L-1) of the extracts showed inhibitory effect all the time. The maximum inhibitory rate of the extracts at concentrations of 10, 20, and 30 g·L-1 was 375%, 393%, and 565%, respectively. After treated with the extracts, part of C. pyrenoidosa cells aggregated, expanded, turned transparent, and finally lysed. The amount of the aggregated cells increased with increasing concentration of the extracts. When treated with 30 g·L-1 of the extracts, C. pyrenoidosa cells largely aggregated, and precipitated as cell pellets to the bottom of the container.

Key words: Acetochlor, Methamidophos, Fungi community, PCR-DGGE, Black soil