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尖角突脐孢菌粗毒素对稗草生理活性的影响

郑伟1;陈勇1;崔海兰2;张宏军3;谭卫军1;余特1   

  1. 1华南农业大学杂草研究室, 广州 510642;2中国农业科学院植物保护研究所 杂草害鼠生物学与治理研究室, 北京 100094;3农业部农药检定所, 北京 100026
  • 收稿日期:2008-02-18 修回日期:1900-01-01 出版日期:2008-09-10 发布日期:2008-09-10

Effects of Exserohilum monoceras crude toxin on the physiological activities of Echinochloa crusgalli.

ZHENG Wei1;CHEN Yong1;CUI Hai-lan2;ZHANG Hong-jun3;TAN Wei-jun1; YU Te1   

  1. 1Weed Science Laboratory, College of Agronomy, South China Agricultural
    University, Guangzhou 510642, China;2Department of Weed and Rode
    nt Biology and Management, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 10094, China;3Institute for Control of agroch
    emicals, Ministry of Agriculture, Beijing 100026, China
  • Received:2008-02-18 Revised:1900-01-01 Online:2008-09-10 Published:2008-09-10

摘要: 以尖角突脐孢菌(Exserohilum monoceras X27) 菌株的粗毒素为材料,通过滤纸法、离体叶片法和盆栽喷雾法,测定了粗毒素对稗草萌芽及生长的抑制作用,并测定了2叶期稗草叶片叶绿素含量、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和相对电导率等4项生理指标。结果表明: 粗毒素浓度为5 g·L-1时,强烈抑制稗草种子的萌芽及胚根胚芽的伸长;4叶期以下稗草的离体叶片全部枯黄;显著影响1、2叶期稗苗的生长,喷施毒素后第7天苗高抑制率均超过20%,第28天地上部分和地下部分鲜质量抑制率均分别超过40%和70%;7 d内,2叶期稗叶的上述4项生理指标受到显著影响:叶绿素含量下降、SOD活性升高、MDA含量升高和相对电导率增大。

关键词: 羊尿斑块, 化学特性, 碳, 氮, 磷, 时间变化

Abstract: The crude toxin was extracted from Exserohilum monoceras X27, and its effects on the inhibition of seed germination, radical growth, embryo growth, and seedling growth of Echinochloa crus-gallias well as the chlorophyll content, superoxide dismutase (SOD) activity, malondiadehyde (MDA) content, and relative electrical conductivity (EEC) of E. crus-galli leaves at 2nd-leaf stage were studied by the methods of filter paper, detached leaf, and spot-spraying. The results showed that the toxin at 5.0 g·L-1 strongly inhibited the seed germination, radical growth, and embryo growth, and made the detached leaves before 4th-leaf stage completely dead. The crude toxin also strongly affected the growth of plants at 1st- and 2ndleaf stages. On the 7th day after spraying toxin, the inhibition rate of seedling height exceeded 20%; while on the 28th day, the mass inhibition rate of above- and underground parts exceeded 40% and 70%, respectively. Within the 7 days of spraying the toxin, the chlorophyll content of 2nd leaf stage plants decreased significantly, while MDA content, SOD activity and EEC were in adverse.

Key words: Sheep urine patches, Soil chemical properties, Carbon, Nitrogen, Phosphorus, Temporal change