欢迎访问《生态学杂志》官方网站,今天是 分享到:

生态学杂志

• 研究报告 • 上一篇    下一篇

金龟子绿僵菌菌株生长环境变量的优化

杨腊英1;甘露2;刘丽2;文明富2;黄俊生1   

  1. 1中国热带农业科学院环境与植物保护研究所, 海南省热带农业有害生物检测监控重点实验室, 海南儋州 571737;2华南热带农业大学农学院, 海南儋州 571737
  • 收稿日期:2007-08-24 修回日期:1900-01-01 出版日期:2008-08-10 发布日期:2008-08-10

Optimization of environmental factors for the growth of Metarhizium anisopliae var. anisopliae strains MA4 and MAlm1.

YANG La-ying1;GAN Lu2;LIU Li2;WEN Ming-fu2;HUANG Jun-sheng1   

  1. 1Key Laboratory for Baleful Biology Detection and Monitor of Tropical Agr
    iculture of Hainan Province, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, Hainan, China; 2Agronomy College, South China University of Tropical Agriculture,
    Danzhou 571737, Hainan, China
  • Received:2007-08-24 Revised:1900-01-01 Online:2008-08-10 Published:2008-08-10

摘要: 金龟子绿僵菌(Metarhizium anisopliae var. anisopliae)是一种广谱的昆虫病原真菌,提高菌株的生长速率及产孢量具有重要的意义。分别对2株金龟子绿僵菌菌株MA4与MAlm1的菌丝生长及次代产孢具有影响的培养温度、培养基初始pH、装液比、光照及微量元素等环境因素进行了测定。经过优化得到2菌株最佳培养条件分别为:MA4菌株在28 ℃、pH 7、装液比为75 ml/250 ml、加入微量元素Mn全光照培养时生长最好、次代产孢量最高;MAlm1菌株在28 ℃、pH 9、装液比为75 ml/250 ml、加入微量元素Cu、黑暗培养时生长最好、次代产孢量最高。

关键词: 水分环境, 空间格局, GIS, 黄土高原

Abstract: Metarhizium anisopliae var. anisopliae is a kind of broad spectrum entomopathogenic fungi, and thus, to increase its growth rate and sporification is of important significance. In this study, the environmental factors affecting hypha growth and second generation sporulation of two M. anisopliae var. anisopliae strains MA4 and MAlm1, such as culture temperature, initial pH of culture medium, entire volume of liquid, illumination, and microelements, were determined. The results showed that the optimal growth and sporification conditions for MA4 strain were 28 ℃, pH 7, 75 ml/250 ml installment, 24 h light/day with Mn addition; while those for MAlm1 strain were 28 ℃, pH 9, 75 ml/250 ml installment, 0 h light/day, and Cu addition.

Key words: Water circumstances, Spatial patterns, GIS, Loess Plateau