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生物造粒流化床污水处理反应器的微生物多样性

袁宏林;刘永军;王晓昌;马美玲;左丽丽   

  1. 西安建筑科技大学环境与市政工程学院西北水资源与环境生态教育部重点实验室,西安 710055
  • 收稿日期:2006-07-14 修回日期:1900-01-01 出版日期:2007-08-13 发布日期:2007-08-13

Microbial diversity in fluidized pellet-bed bioreactor.

YUAN Hong-lin;LIU Yong-jun;WANG Xiao-chang;MA Mei-ling;ZUO Li- li   

  1. Key Laboratory of Northwest Water Resources,Environment and Ecology of Education Ministry, College of Environmental and Municipal Engineering, Xi’an University of Achitecture & Technology, Xi’an 710055, China
  • Received:2006-07-14 Revised:1900-01-01 Online:2007-08-13 Published:2007-08-13

摘要: 为了研究生物造粒流化床污水处理反应器颗粒污泥的微生物种群多样性,分别从生物造粒流化床10、60和110 cm处取颗粒污泥,通过细胞裂解直接提取颗粒污泥细菌基因组DNA,PCR扩增后经变性梯度凝胶电泳(DGGE)分离,获得微生物群落的DNA特征指纹图谱,对特征条带进行序列测定及序列同源性分析。16S rRNA序列分析表明,获得的18个OTUs均属于细菌域,其中61%属于变形菌,17%属于放线菌,11%属于低G+C革兰氏阳性菌,11%属于其它未知细菌。

关键词: 土壤有机质动态, 土壤有机质模型, 扩散作用, 平移作用, 陆地生态系统

Abstract: To study the microbial diversity of granular sludge in fluidized pellet-bed (FPB) bioreactor, the sludge at different heights (10, 60 and 110 cm) of FPB was sampled, and the genomic DNA of microbial community was extracted directly. After purifying the genomic DNA, 16S rRNA genes were amplified, and the amplified DNA fragments were separated by denaturing gradient gel electrophoresis (DGGE). The characteristic bands of DGGE were sequenced, and the phylogenetic diversity of microbes in the FPB bioreactor was surveyed based on the homology analysis of 16S rRNA sequences. The results indicated that the obtained 18 operational taxonomic units (OTUs) all belonged to Eubacteria, of which, 61% was Proteobacteria, 17% was Actinobacteria, 11% was low G+C gram-positive bacterium, and 11% belonged to other bacterial branches.

Key words: SOM dynamics, SOM model, Diffusion motion, Translation motion, Terrestrial ecosystem