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益母草不同组分的抑螺效果及对钉螺酯酶同工酶的影响

胡兴宜1;唐万鹏1;王万贤2;张旭东3   

  1. 1湖北省林业科学研究院, 武汉 430079;2湖北大学生命科学学院, 武汉 430062;3中国林业科学院林业研究所, 北京 100091
  • 收稿日期:2006-06-02 修回日期:1900-01-01 出版日期:2007-05-08 发布日期:2007-05-08

Effects of Leonurus heterophyllus extracts on Oncomelania hupensis and its esterase isoenzyme.

HU Xing-yi1; TANG Wan-peng1; WANG Wan-xian2; ZHANG Xu-dong3   

  1. 1Hubei Academy of Forestry, Wuhan 430079, China;2School of Life Science, Hubei University, Wuhan 430062, China;3Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
  • Received:2006-06-02 Revised:1900-01-01 Online:2007-05-08 Published:2007-05-08

摘要: 通过水浸液灭螺实验,对益母草的根、茎、叶水浸液及益母草水苏碱水溶液的杀灭钉螺作用进了初步研究。结果表明:各处理均有较好的灭螺效果,但毒效较氯硝柳胺略慢,益母草各部分灭螺效果的顺序为叶>茎>根;采用同工酶电泳技术检测了益母草水浸液处理钉螺时对钉螺酯酶同工酶的影响,处理1~2 d后样品的酶活高于对照组,处理3~4 d后的酶活则大大减弱,在这一过程中,有时出现新的酶带,有时又有酶带消失,酶带的变化主要在正极区,与正常的病 理反应完全一致。

关键词: 土壤有机质, 模型, 比较

Abstract: The study showed that both the aqueous and the stachydrine extracts of Leonurus heterophyllus’s root, stem and leaf had good effect on controlling Oncomelania hupensis, but their toxic activity was somewhat slower than that of niclosamide. The effect was in the sequence of leave>stem>root. After treated with the aqueous extracts of L. heterophyllus, the esterase isoenzyme activity of O. hupensis was higher than that of the control in 1-2 days, but decreased markedly in 3-4 days. During this process, some new bands of the enzyme appeared, and some bands disappeared. This change was mainly occurred in positive area, which accorded with the normal pathological reaction.

Key words: Soil organic matter, Models, Comparison