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太湖梅梁湾冬季浮游细菌的多样性

吴鑫;奚万艳;杨虹   

  1. 上海交通大学生命科学与技术学院, 上海 200240
  • 收稿日期:2006-03-07 修回日期:2006-06-03 出版日期:2006-10-10 发布日期:2006-10-10

Bacterioplankton diversity in Meiliang Bay of Taihu Lake in winter

WU Xin;XI Wanyan;YANG Hong   

  1. School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China
  • Received:2006-03-07 Revised:2006-06-03 Online:2006-10-10 Published:2006-10-10

摘要: 运用分子生物学方法对太湖梅梁湾2005年3月冬季水体中的浮游细菌多样性进行了研究。提取水样中细菌基因组总DNA,以细菌的16S rDNA通用引物进行PCR扩增,扩增产物经分子克隆、酶切归类分析、测序和序列分析,对水样中的细菌群落建立了针对16S rDNA的克隆文库和系统发生树。基于细菌16S rDNA克隆文库和系统发生树的分析,结果表明,该水域的优势细菌类群为拟杆菌(Cytophaga Flavobacterium Bacteroides, 44.3%)和β、γ变形杆菌(Proteobacteria; β-Proteobacteria, 25.3%和γ-Proteobacteria, 27.8%),其中大部分细菌与黄杆菌属(Flavobacterium)、假单胞菌属(Pseudomonas)和食酸菌属(Acidovorax)细菌的关系密切。与国外富营养化湖泊的研究报道相比较,γ-Proteobacteria类群为水体中的优势类群,是严重富营养化的太湖在冬季所具有的独特种群结构特征。

关键词: 褐土, 秸秆还田, 促腐条件, 微生物量, 动态变化

Abstract: In this paper, the bacterioplankton diversity in the water body of Meiliang Bay in March, 2004 was studied by using the techniques of molecular biology, including the extraction of bacterial DNA, PCR amplification of bacterial 16S rDNA by universal primers, molecular clone, RFLP analysis, sequencing of 16S rDNA fragments, and sequence analysis. The clone library aimed at 16S rDNA and the phylogenetic tree were constructed. The analysis of sequencing results based on 16S rDNA indicated that the dominant bacterial groups were Cytophaga-Flavobacterium-Bacteroides (CFB) plylum (44.3%), β-Proteobacteria (25.3%), and γ-Proteobacteria (27.8%), and most of the bacteria were closely related to genera Flavobacterium, Pseudomona and Acidovorax. Compared with the eutrophicated lakes abroad, Tailu Lake, the heavily eutrophicated lake in China, had the unique characteristic of γ-Proteobacteria population dominated in winter.

Key words: Cinnamon soil, Returning straw into field, Promoted decay condition, Microbial biomass, Dynamic change