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杂色藻类硅藻和金藻的77 K荧光特性

桑敏;李爱芬;徐宁;刘太胜;段舜山   

  1. 暨南大学水生生物研究中心,广州 510632
  • 收稿日期:2006-01-04 修回日期:2006-05-20 出版日期:2006-09-10 发布日期:2006-09-10

Characteristics of fluorescence emission spectrum at 77 K of diatom and chrysophyta in Chromaphytae

SANG Min; LI Aifen; XU Ning; LIU Taisheng; DUAN Shunshan   

  1. Institute of Hydrobiology, Ji’nan University, Guangzhou 510632, China
  • Received:2006-01-04 Revised:2006-05-20 Online:2006-09-10 Published:2006-09-10

摘要: 实验测定了7种杂色藻包括:4种硅藻三角褐指藻(Phaeodactylum tricornutum)、牟氏角毛藻(Cheatoceros mulleri)、中肋骨条藻(Skeletonema costatum)、尖刺拟菱形藻(Pseudonitzschia pungens)和3种金藻湛江等鞭金藻(Isochrysis zhanjiangensis)、绿色巴夫藻(Pavlova viridis)、球形棕囊藻(Phaeocystis globosa)的77 K荧光光谱,并与其它杂色藻以及高等植物菠菜进行了比较。结果表明,4种硅藻和3种金藻的77 K低温荧光发射光谱相似,只有一个明显的荧光发射主峰位于684~686 nm,类囊体膜的荧光光谱中在700 nm附近有个不明显的肩峰,它们均没有高等植物作为PSⅠ特征的730 nm长波荧光发射峰,这个结果与杂色藻类褐藻的荧光特性一致。说明,杂色藻类PSⅠ缺少730 nm长波荧光峰可能具有其普遍性,预示杂色藻类在PSⅠ结构以及能量传递等方面与高等植物具有很大的差异。

关键词: 蒙古裸腹氵蚤, 内禀增长率, 温度, 盐度

Abstract: This paper determined the fluorescence emission spectrum at 77 K of four diatoms Phaeodactylum tricornutum,Cheatoceros mulleri,Skeletonema costatum and Pseudo-nitzschia pungens and three chrysophytas Isochrysis zhanjiangensis, Pavlova viridis and Phaeocystis globosa, with that of other algae in Chromaphytae and of higher plant Spinacia oleracea compared. The results showed that these four diatoms and three chrysophytas had a similar fluorescence emission spectrum at 77 K,i.e., a significant major fluorescence emission at 684~686 nm and a not significant shoulder peak near 700 nm from thylakoid membrane, and had no fluorescence emission at 730 nm, the characteristic of PS I in higher plants, which were consistent with the spectral properties of brown algae in Chromaphytae. It was suggested that the lack of longwavelength fluorescence emission at 730 nm from PS I in Chromaphytae might be of common, predicting the great differences of PS I structure and energy transfer between Chromaphytae algae and higher plants.

Key words: Moina mongolica, Intrinsic increasing rate(rm), Temperature, Salinity