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不同植物群落下酸化尾矿养分状况及土壤酶活性

任冠举;孙庆业;安树青;李远;李旭   

  1. 南京大学生命科学学院, 南京 210098
  • 收稿日期:2005-07-18 修回日期:2005-09-30 出版日期:2006-04-10 发布日期:2006-04-10

Nutrient status and enzyme activities of acidified tailings under different plant communities

REN Guanju;SUN Qingye;AN Shuqing;LI Yuan,LI Xu   

  1. School of Life Science,Nanjing University,Nanjing 210098, China
  • Received:2005-07-18 Revised:2005-09-30 Online:2006-04-10 Published:2006-04-10

摘要: 调查不同植被群落下铜尾矿的养分状况及土壤酶活性的变化动态,通过常规方法测定了尾矿的pH和电导率,以及有机质,总氮,有效磷,速效钾的含量。用底物反应法测定了过氧化氢酶,芳基硫酯酶,脲酶,酸性磷酸化酶和脱氢酶的活性。结果表明,与酸化的裸露尾矿相比,定居其上的芦苇(Phragmites australis)、狗牙根(Cynodon dactylon)和双穗雀稗(Paspalum distichum)均能显著增加尾矿基质pH值(从3.6上升到5.4),降低电导率,减缓尾矿酸化过程。芦苇和狗牙根能够显著提高尾矿基质中N、K和有机质含量(P<0.05),增加尾矿中的养分。3种植物群落下有效磷较裸露尾矿没有显著增加。植物的定居显著提高了尾矿中过氧化氢酶、芳基硫脂酶和脲酶的活性(P<0.05);但酸性磷酸化酶和脱氢酶活性没有显著提高。研究表明,植物对尾矿有明显的改良作用,而且芦苇和狗牙根优于双穗雀稗。在各种植被条件的尾矿中除酸性磷酸化酶以外的其它实验土壤酶活性均与尾矿中有机质和总氮呈极显著正相关(P<0.01)。

关键词: 生物多样性, 稳定性, 人工群落

Abstract: Nutrient status and enzyme activities of acidified tailings in the Tongling copper mine area under Phragmites australis,Cynodon dactylon,and Paspalum distichum communities were studied in this paper.The pH value,electric conductivity,and contents of organic matter,total nitrogen,available phosphorus and available potassium in the tailings were determined by routine methods,and the activities of catalase,arylsulfatase,urase,acid phosphomonoesterase and dehydrogenase were determined by substratereaction method.Comparing with acidified bare tailings,the tailings under P.australis,C.dactylon,and P.distichum communities had higher pH value and lower electric conductivity,and their acidification process was slowed down.P.australis and C.dactylon communities could increase the total nitrogen,available potassium and organic matter contents of the tailings significantly (P<0.05),but the available phosphorus content of the tailings under the three natural plant communities did not have any change.The establishment of plant communities also increased the catalase,arylsulfatase and urase activities of the tailings significantly (P<0.05),but the activities of acid phosphomonoesterase and dehydrogenase did not increased significantly.Under plant communities,the tailings were meliorated,and P.australis and C.dactylon communities had better effects than P.distichum community.The activities of test enzymes except acid phosphomonoesterase had a significantly positive correlation (P<0.01) with the contents of organic matter and total nitrogen,which provided scientific evidence to use enzyme activities as the indicators of the tailings’ properties.

Key words: Species diversity, Managed ecosystem, Stabilit