Bt玉米秸秆Bt蛋白的土壤降解及其拟合模型的比较

生态学杂志 ›› 2005, Vol. ›› Issue (9): 1063-1067.

Bt玉米秸秆Bt蛋白的土壤降解及其拟合模型的比较

王建武, 冯远娇

华南农业大学热带亚热带生态研究所, 广州, 510642

收稿日期:2004-07-19 修回日期:2005-01-27 出版日期:2005-09-10
通讯作者: 王建武,男,1966年生,博士,教授.主要从事转基因作物安全性评价与分子生态学研究,发表论文50余篇.E-mail:wangjw@scau.edu.cn
基金资助:
国家自然科学基金项目(3027027);广东省自然科学基金资助项目(000569,021043)

Degradation of Bt protein in soil released from Bt corn stalks and comparison of its simulation models

WANG Jianwu, FENG Yuanjiao

Institute of Tropical and Subtropical Ecology, South China Agricultural University, Guangzhou 510642, China

Received:2004-07-19 Revised:2005-01-27 Online:2005-09-10

摘要/Abstract

摘要： 研究4种Bt玉米(34B24、NK58-D1、R×601RR/YG和农大61)秸秆分解释放Bt蛋白的土壤降解动态,分别应用一级动力学反应方程、双指数模型和移动对数模型进行了拟合。结果表明,4种Bt玉米Bt蛋白的土壤降解均呈现前期负指数大量快速降解和中后期极少量稳定两个阶段,双指数模型与移动对数模型的拟合结果比一级动力学反应方程更符合实际。判断模型拟合精度时不能只用拟合结果的统计指标值来确定,图示对比实测值与模拟值的差异是避免应用统计学拟合精度指标很高但并不符合实际的模型错误估算DT₅₀的有效办法。在研究转基因作物秸秆中Bt蛋白土壤降解规律时,应该根据其土壤降解前期较快的特点,增加前期取样的次数,以便更好地对模拟模型求得的安全性评价关键参数DT₅₀的准确性进行科学判断。

关键词: 产甲烷菌, 甲烷氧化菌, 硝化细菌, 反硝化细菌, CH₄, N₂O

Abstract: The soil degradation of Bt protein from stalks of 4 Bt corn hybrids(34B24,NK58-D1,R?601 RR/YG and Nongda61)were compared and the first-order kinetic model,biexponential model and shift-log model were used to fit the degradation data set in this study.The results showed that the degradation of Bt protein of all 4 hybrids in soil demonstrated a two-stages pattern,a rapid decline in 14 days and then stable at low concentration for a long time.Both the biexponential model and shift-log model fitted the decay pattern of Bt protein much better than the first-order kinetic model.However,model selection should not only be based on the statistic significant index of the model fitting,but also on the deviation between each simulated value and observed value.It was necessary to increase sampling frequently in the rapid degradation stage so as to make a scientific judgment about the accuracy of DT₅₀ and DT₉₀ calculated from models.

Key words: Methanogens, Methanotrophs, Nitrifiers, Denitrifiers, CH₄, N₂O

中图分类号:

X705

王建武, 冯远娇. Bt玉米秸秆Bt蛋白的土壤降解及其拟合模型的比较[J]. 生态学杂志, 2005, (9): 1063-1067.

WANG Jianwu, FENG Yuanjiao. Degradation of Bt protein in soil released from Bt corn stalks and comparison of its simulation models[J]. cje, 2005, (9): 1063-1067.

参考文献

[1] 王建武,冯远娇,骆世明.2003.Bt玉米抗虫蛋白表达的时空动态及其土壤降解研究[J].中国农业科学,36(11):1279～1286.
[2] Frey M, Stettner C, Pare PW. 2000. An herbivor elicitor activates the gene for indole emission in maize[J]. Proceed National Academy Sci. USA, 97:14801 ～ 14806.
[3] Gierl A, Frey M. 2001. Evolution of benzoxazinone biosythesis and indole production in maize[J]. Planta, 213: 493 ～ 498.
[4] Herman RA, Scherer PN, Wolt JD. 2002. Rapid degradation of a binay, PS149B1, δ-endotoxin of Bacillus thuringiensisin soil,and a novel mathematical model for fitting curve-linear decay[J].Environ. Ent . , 31(2) :208～214.
[5] Herman RA, Wolt JD, Halliday WR. 2002. Rapid degradation of the Cry 1F insecticidal crystal protein in soil[J]. J. Agric. Food Chem., 50:7076～7078.
[6] Hopkins, DW, Gregorich EG. 2003. Detection and decay of the Bt endotoxin in soil from a field trial with genetically modified maize[J]. Europ . J . Soil Sci., 54: 793 ～ 800.
[7] Palm CJ, Schallet Dl, Donegan KK, et al. 1996. Persistence in soil of transgenic plants produced Bacillus thuringiensis var.kurstaki δ-endotoxin[J]. Can. J . Microbiol . , 42:1258～ 1262.
[8] Sach ES, Benedict JH. 1996. Pyramiding cry Ⅰ A insecticidal protein and terpenoids in cotton to resist tobacco budworm[J]. Environ. Ent., 25:1 257～ 1 266.
[9] Sims SR, Holden LR. 1996. Insect bioassay or detemining soil degradation of Bacillus thuringiensis ssp. Kurstaki cryⅠA (b)protetn in com tissue[J]. Environ. Ent., 25:659～664.
[10] Sims SR, Ream JE. 1997. Soil inactivation of the Bacillus huringiensis ssp. Kurstaki cryⅡA insecticidal protein within transgenic cotton tissue; laboratory microcosm and field studies [J]. J. Agric. Food Chem., 45:1502 ～ 1505.
[11] SPSS Inc. 2000. Sigma Plot 2000 Programming Guide[M].Chicagoi: IL. USA. SPSS Inc. 147～ 250.
[12] Stotzky G. 2000. Persistence and biological activity in soil of insecticidal proteins from Bacillus thuringiensis and of bactetial DNA bound on clays and humic acids[J]. J. Environ. Qual.,29: 691～ 705.
[13] Wolt JD, Nelson HP, Cleveland CB, et al. 2001. Biodegradation kinetics for pesticide exposure assessment [J]. Rev. Environ.Contam. Toxicol., 169:123 ～ 164.
[14] Zwahlen C, Hilbeck A, Gugerli P, et al. 2003. Degradation of the Cry1Ab protein within transgenic Bacillus thuringiensis corn tissue in the field[J]. Molec. Ecol., 12:765～775.

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