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生态学杂志 ›› 2005, Vol. ›› Issue (7): 768-772.

• 研究报告 • 上一篇    下一篇

微囊藻和栅列藻光吸收特性的实验研究

吴生才1,2,3, 张运林1,2, 陈伟民1   

  1. 1. 中国科学院南京地理与湖泊研究所太湖生态系统研究站, 南京, 210008;
    2. 中国科学院研究生院, 北京, 100039;
    3. 盐城师范学院, 盐城, 224002
  • 收稿日期:2004-03-05 修回日期:2004-05-08 出版日期:2005-07-10
  • 通讯作者: 张运林
  • 基金资助:
    中国科学院知识创新工程项目(KZC-XSW-12);国家自然科学基金资助项目(40203007和30200032).

On the absorptive characteristics of Microcystis and Scenedesmus

WU Shengcai1,2,3, ZHANG yunlin1,2, CHEN Weimin 1   

  1. 1. Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China;
    2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China;
    3. Yancheng Teachers College, Yancheng 224002, China
  • Received:2004-03-05 Revised:2004-05-08 Online:2005-07-10

摘要: 在室内模拟槽中,以人工灯为光源,分别检测不同密度的微囊藻和栅列藻细胞悬液的光谱衰减,检测各样品的叶绿素含量和过滤后清液的溶解性有机碳(DOC)含量,并在紫外分光光度计上检测DOC光谱吸收,然后换算成吸收系数.再根据近似的线性加和关系计算出细胞悬液中各组分(藻细胞、水、DOC)的吸收系数.结果表明,微囊藻和栅列藻光衰减的波谱特征相似,在3个较高密度下的衰减系数的变化趋势相近,其衰减系数贡献率的平均值分别为84.9%和88.1%.将衰减系数除以各自的叶绿素浓度即可得到叶绿素的比吸收系数,两种藻3个较高密度的比吸收系数的平均值具有相似的波谱特征,在380~500、660~690 nm两个波段有较明显的峰值,栅列藻和微囊藻在435 nm的比吸收系数峰值分别为0.1118、0.0413(m2·mg-1chla),在670 nm处的峰值分别为0.075、0.032(m2·mg-1chla).但两者的比吸收系数差异较大,三个密度栅列藻在340~800 nm的平均比吸收系数是0.0573(m2·mg 1chla),而微囊藻仅为0.0234(m2·mg-1chla),且微囊藻的所有波长的比吸收系数均小于栅列藻.根据这些参数来讨论两种藻类在分光衰减中的贡献率和光谱特征方面的差异及其生态学意义,以及在浮游植物的遥感监测方面的参考价值.

关键词: P流失, 模拟降雨, 坡地, 土壤翻耕, 土壤压实

Abstract: The diffuse attenuation coefficient (Kd) of 6 densities of Microcystis and Scenedesmus cell media were measured in simulative column indoor.The specific absorption coefficient (SAC) and the contribution of cell to total Kd were calculated after testing the content of chla.The absorption coefficient of dissolved organic carbon (DOC) was derived from the conversion of absorbance value in spectrophotometer.The result indicated that the spectrum characteristic of 2 kinds of algal cell in higher densities was similar and the Kd at 3 higher density of both algae had the same trend.The contribution to Kd was 84.95% (Microcystis) and 88.15% (Scenedesmus), respectively.Their mean SAC in 3 higher densities demonstrated parallel spectrum property.There were two obvious peaks in 380~500nm and 660~690nm with the corresponding peak values 0.1118,0.0413(m2·mg-1chla) at 435 nm and 0.075,0.032(m2·mg-1chla) at 670 nm.Scenedesmus cell has much higher SAC than Microcystis cell.The mean SAC of 3 densities of Scenedesmus in 340~800 nm was 0.0573(m2·mg-1 chla),while that of Microcystis was 0.0234(m2·mg-1 chla).The ecological significance of the differences and the potential utilization in remote sensing monitoring were discussed.

Key words: Phosphorus loss, Simulated rainfall, Slope land, Soil tillage, Soil compaction

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