重金属镉抗性菌株的筛选及其对镉活化作用的研究

生态学杂志 ›› 2005, Vol. ›› Issue (11): 1357-1360.

重金属镉抗性菌株的筛选及其对镉活化作用的研究

夏娟娟, 盛下放, 江春玉

南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室, 南京, 210095

收稿日期:2005-03-30 修回日期:2005-06-28 出版日期:2005-11-10
通讯作者: 盛下放
基金资助:
国家自然科学基金项目资助(40371070)

Screening of cadmium-resistant strains and their effects on cadmium activation

XIA Juanjuan, SHENG Xiafang, JIANG Chunyu

Key Laboratory of Microbiological Engineering of Agricultural Environment of Agriculture Ministry, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China

Received:2005-03-30 Revised:2005-06-28 Online:2005-11-10

摘要/Abstract

摘要： 通过在培养基中加入一定浓度的Cd²⁺,从土壤中分离筛选出2株具有较强镉抗性及产酸能力的细菌JL-4和JC-9。经初步鉴定,JL-4和JC-9分别属于芽孢杆菌属(Bacillus sp.)和黄单胞菌属(Xan-thomonas sp.)。将菌株JL-4和JC-9接种到添加不溶性镉(100 mg·L^-1)的培养液中28℃培养48 h,接菌处理比不接菌对照培养液中有效镉含量分别增加1 392.3%和1 410.8%,培养液pH分别降低了2.87和3.18。将JL-4和JC-9分别接入含不同浓度重金属镉(100、200 mg·kg^-1)的土壤中28℃培养20 d,接菌处理比不接菌对照土壤中有效镉含量分别增加17.02%～100%(JL-4)和36.17%～61.80%(JC-9)。菌株JL-4和JC-9的最适生长温度均为28℃,最适pH值分别为7和8。除对镉具有抗性外,两菌株对重金属铅、铜、镍、锌也具有一定抗性。

关键词: 森林植被, 碳储量, 黑龙江省

Abstract: In this paper,cadmium-resistant bacteria were isolated from heavy metals-contaminated soils through agar plate containing Cd²⁺ as 3CdSO₄·8H₂O.The strains JL-4 and JC-9 were identified as Bacillus sp.and Xanthomonas sp.,respectively,based on their morphological,physical,and biochemical characteristics.In the liquid medium containing Cd²⁺(100 mg·L^-1) and inoculated with JL-4 or JC-9,the available Cd²⁺ content in supernatant was increased by 1 392.3%(JL-4) or 1 410.8%(JC-9),compared with the control,and the pH was decreased to 2.87(JL-4) or 3.18(JC-9).Soil cadmium(100,200 mg·kg^-1) activation experiments showed that the available Cd²⁺ content in soil inoculated with JL-4 or JC-9 was increased by 17.02%～100%(JL-4) or 36.17%～61.80%(JC-9),compared with the control.The optimum temperature for JL-4 and JC-9 was 28 ℃,and the optimum pH for them was 7 and 8,respectively.The two strains could also be resistant to Pb²⁺(800 mg·L^-1),Cu2+(50 mg·L^-1),Ni²⁺(50 mg·L^-1),and Zn²⁺(200 mg·L^-1).

Key words: Forest vegetation, Carbon storage, Heilongjiang Province

中图分类号:

夏娟娟, 盛下放, 江春玉. 重金属镉抗性菌株的筛选及其对镉活化作用的研究[J]. 生态学杂志, 2005, (11): 1357-1360.

XIA Juanjuan, SHENG Xiafang, JIANG Chunyu. Screening of cadmium-resistant strains and their effects on cadmium activation[J]. cje, 2005, (11): 1357-1360.

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