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生态学杂志 ›› 2004, Vol. ›› Issue (3): 68-71.

• 研究报告 • 上一篇    下一篇

长白山三种赤杨根瘤内Frankia基因多样性的REP分析

唐晓萌1, 代玉梅1, 熊智2, 曹军1   

  1. 1. 中国科学院沈阳应用生态研究所, 沈阳, 110016;
    2. 西南林学院, 昆明, 650224
  • 收稿日期:2003-04-30 修回日期:2003-07-02 出版日期:2004-06-10
  • 基金资助:
    国家重点基础研究发展规划项目(NO001CB1089);中国科学院沈阳应用生态研究所创新项目(C11AG-SCXZY0106);中国科学院长白山森林生态系统定位研究站资助

Genetic diversity analysis of Frankia strains in nodules of three Alnus species from Changbai Mountain by REP fingerprinting

TANG Xiaomeng1, DAI Yumei1, XIONG Zhi2, CAO Jun1   

  1. 1. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
    2. Southwest Forestry College, Kunming 650224, China
  • Received:2003-04-30 Revised:2003-07-02 Online:2004-06-10

摘要: 利用REPPCR方法研究了长白山不同海拔处3种赤杨根瘤内Frankia菌基因多样性。结果表明,赤杨根瘤内Frankia菌存在丰富的基因多样性。宿主和海拔因素共同影响长白山赤杨属Frankia菌的基因多样性:东北赤杨Frankia菌的宿主专一性很强,西伯利亚赤杨和色赤杨的Frankia菌的有较近的亲缘关系。不同海拔的同种宿主共生的Frankia菌基因类型不同,相同海拔的西伯利亚赤杨和色赤杨的Frankia菌出现宿主混乱现象.

关键词: 黑土, 有机肥, 脲酶活性, 动态变化, 土壤养分

Abstract: The genetic diversity of the Frankia strains in the nodules of Alnus tinctoria,A.mandshurica and A.sibirica growing at different altitude in Changbai Mountain was studied by REP-PCR fingerprinting.The REP-PCR fingerprint patterns revealed that uncultured Frankia strains tested had a high genetic diversity.Host and altitude factors influenced the genetic diversity.Frankia strains from A.mandshurica showed greater specialization toward the host,and Frankia stains from A.sibirica and A.tinctoria had a closer affinity.The Frankia stains from the same alder distributing at different altitude belonged to different REP patterns,and the Frankia stains from A.sibirica and A.tinctoria at the same altitude showed promiscuity.

Key words: Black soil, Farmyard manure, Urease activity, Dynamic change, Soil nutrient

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