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生态学杂志 ›› 2004, Vol. ›› Issue (3): 128-130.

• 研究简报 • 上一篇    下一篇

苜蓿化感作用的初步研究

朱晓红1,2, 王朋1, 梁文举1, 姜勇1, 臧树良2   

  1. 1. 中国科学院沈阳应用生态研究所, 沈阳, 110016;
    2. 辽宁大学化学科学与工程学院, 沈阳, 110036
  • 收稿日期:2003-10-20 修回日期:2004-01-13 出版日期:2004-06-10
  • 基金资助:
    国家自然科学基金(30270271);中国科学院沈阳生态实验站开放基金资助项目(SYZ0301)

Preliminary study on allelopathic potential of alfalfa (Madicago sadiva L.)

ZHU Xiaohong1,2, WANG Peng1, LIANG Wenju1, JIANG Yong1, ZANG Shuliang2   

  1. 1. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China;
    2. College of Chemistry and Chemical Engineering, Liaoning University, Shenyang 110036, China
  • Received:2003-10-20 Revised:2004-01-13 Online:2004-06-10

摘要: 利用生物测定法和气相色谱/质谱(GC/MS)联用技术对中国北方广为种植的紫花苜蓿的化感作用进行了初步研究。结果表明,紫花苜蓿全草的水和95%乙醇浸提液对玉米和小麦种子萌发均有显著的抑制作用,进一步用柱层析对苜蓿浸提液进行了初步分离,分离组份对玉米和小麦种子萌发的影响有所变化。抑制玉米萌发的活性物质主要在弱极性的二氯甲烷组份中,而抑制小麦萌发的活性物质不仅存在于二氯甲烷组份中,也存在于水中。采用GC/MS技术,初步推测苜蓿的化感物质是酚和皂甙两类化合物.

Abstract: The allelopathic potential of alfalfa (Madicago sadiva L.) was preliminarily studied by using the bioassay and GC/MS.The results showed that the germination of maize,wheat and soybean were significantly inhibited by aqueous and 95% ethanol extracts from alfalfa.Further isolation of alfalfa extracts was carried out with column chromatography.Inhibition of the isolated fractions differed from that of the extracts.Allelochemicals of alfalfa on maize germination were low polarity,while allelochemicals of alfalfa on wheat germination were both low and high polarity.The potential allelochemicals of alfalfa were phenolics and saponins identified with GC/MS.

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