RAPD技术及其在哺乳动物生态学研究中的应用

摘要/Abstract

Abstract: Random amplified polymorphic DNA (RAPD) has been widely applied in taxonomy, genetic variation etc. because of its convenience,rapid and sensitivity. Its principle, influential factor and application in mammal ecological research are introduced and discussed in this paper.

Key words: ecological stoichiometry, climate change, terrestrial plant, carbon, nitrogen, and phosphorus.

包惠芳, 徐宏发, 陆厚基. RAPD技术及其在哺乳动物生态学研究中的应用[J]. 生态学杂志, 2000, (2): 72-73,74,75,78.

Bao Huifang, Xu Hongfa, Lu Houji. Random Amplified Polymorphic DNA (RAPD) and its Application in Mammal Ecological Research[J]. cje, 2000, (2): 72-73,74,75,78.

参考文献

[1] Williams, J. G. K. et al. DNA polymorphisms amplified by arbitrary primer are useful as genetic markers. Nucleic Acids Res.,1990,18(22):6531-6535.
[2] Welsh, J. and Mcclelland, M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res.,1990,18(24):7213-7218.
[3] 汪小全,邹喻苹. RAPD应用于遗传多样性和系统学研究中的问题. 植物学报,1996,38(12):954-962.
[4] Ho Chai-ling et al. Molecular characterisation of Sargassum polycystum and S.Siliquosum (Phaeophyta) by polymerase chain reaction (PCR) using random amplified polymorphic DNA(RAPD) primers. Journal of Applied Phycology,1995,7:33-41.
[5] Skroch,P. V. et al. RAPD protocol for the air themo-cycler. Rapidcyclist,1992,1:9.
[6] 陈永久,张亚平. 随机扩增多态DNA影响因素的研究. 动物学研究,1997,18(2):221-227.
[7] 李向峰,隋正红. RAPD技术在龙须菜遗传多样性研究中的应用. 青岛海洋大学学报, 1998,28(2):293-298.
[8] Kubelik, A.R. High-GC primers are useful in RAPD analysis of fungi.Curr Genet, 1995 ,28(4): 384-389.
[9] 秦树臻等. 几种动物RAPD指纹图谱反应条件的分析. 南京师大学报(自然科学版),1996,19(2):66-69.
[10] Schierwater, B. et al. Different thermostable DNA polymerases mayamplify different RAPD products. Nucleic Acids Research,1993,20(19):4647-4648.
[11] 兰宏等. 滇金丝猴的随机扩增多态DNA与遗传多样性分析. 中国科学(C辑),1996,26(3):244-249.
[12] Hidetoshi, B. et al. Assessment of Genetic Variation　　within Population of Sika Deer in Japan by Analysis of Randomly Amplified Polymophic DNA (RAPD). Zoological Science,1995,12:669-673.
[13] Chung,E.R. et al. Analysis of DNA polymorphisms and genetic characteristics in Holstein dairy cattle using RAPD-PCR technique. Korean Jounal of Animal Science,1995,37(5):455-466.
[14] Kantanen,J. et al. Random amplified polymorphic DNA in cattle and sheep: application for detecting genetic variation. Anim-Genet.,1995,26(5):315-320.
[15] 陈永久,张亚平. 中国貉随机扩增多态DNA及其亚种分化关系. 遗传学报,1998,5(1):16-21.
[16] Cho,B.W. et al. Development of RAPD Marker Specific for Korean Cattle(HANWOO). Korean Journal of Animal Sciences, 1995,36(3):263-270.
[17] Bailey,E.et al. Comparison of thoroughbred and Arabian horses using RAPD markers. Animal Genetics,1994,25(sup.1):105-108.
[18] Cargill,S,L. et al. Development of a Species-specific marker using　RAPD analysis to distinguish between sheep and goats. Animal Biotechnology,1995, 6(2):93-100.
[19] 林峰,杨玉华. 应用RAPD技术对大熊猫分类地位的探讨. 兽类学报,1997,17(3) :161-164.
[20] Welsh, J. et al. Polymorphisms generated by arbitrary primed PCR in the mouse application to strain identification and genetic mapping. Nucleic Acids Researsh,1991, 19(2):303-306.
[21] Landry,P.A. et al. RAPD problems in phylogenetics. Zoologica Scripta, 1996, 25(4):283-290.
[22] Paran,et al. Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Therogical Applied Genetics,1993, 85:985-993.
[23] Dedryver,F. et al. Molecular markers linked to the leaf rust resistance gene Lr24 in different wheat cultivars. Genome,1996,39(5):830-835.
[24] Naqvi,N.I. et al. Development of a sequence characterized amplified region(SCAR)based indirect selection method for a dominant blast-resistance gene in rice. Genome,1996,39(1):26-30.
[25] Bhat,K.V. et al.DNA profiling of banana and plantain xultivars using random amplified polymorphic DNA(RAPD) and restriction fragment length polymorphism(RFLP) markers. Electrophoresis,1995,16(9):1736-1745.
[26] Paffetti,D. et al. DNA fingerprinting by random amplified polymorphic DNA and restriction fragment length polymorphism is useful for yeast typing. Res-Microbiol,1995,146(7):587-594.
[27] Whisson,S.C. et al . Phytophthora sojae avirulence genes,RAPD,and RFLP markers used to construct a detailed genetic linkage map. Mol.Plant.Microbe.Interact,1995,8(6):988-995.
[28] Kappe,A.L. et al. Genetic variation in Phoca vitulina (the harbour seal) revealed by DNA fingerprinting and RAPDs. Heredity,1995,74(Pt6): 647-653.